Journal: Cancer discovery

Article Title: A pre-existing rare PIK3CA E545K subpopulation confers clinical resistance to MEK plus CDK4/6 inhibition in NRAS melanoma and is dependent on S6K1 signaling

doi: 10.1158/2159-8290.CD-17-0745

Figure Lengend Snippet: PIK3CAE545K increases pS6 expression levels in patient samples A) Representative pS6 immunohistochemistry and hematoxylin and eosin staining in the patient’s samples: pre-treatment (E545K+ and E545K− regions, n=3 and n=4 respectively), on-anti-PD1 (n=2), and post-PD1-progression (bowel metastasis, n=3); B) Quantification of the pS6 IHC staining signal. Asterisks (*) represent p<0.05 in unpaired t-test vs Pre-treatment group. Error bars represent SEM. C) Schematic of proposed signal transduction pathways culminating in S6- and Rb-mediated control of cell proliferation. Bars represent 100 μm.

Article Snippet: Primary antibodies used were: Anti mTOR (#2983), Anti-phospho-S2448-mTOR (#5536), Anti-p70-S6 Kinase (#9202), Anti phospho-T389-p70-S6 Kinase (#9234), Anti-AKT (#4691), Anti phospho-S473-Akt (#4060), Anti-Ribosomal-Protein-S6 (#2317), Anti phospho-S235/236-Ribosomal-Protein-S6 (#4858), Anti-Rb (#9309), Anti-phospho-S780-Rb (#8180), Anti-4EBP1 (#9644), Anti-phopsho-T37/46-4EBP1(#2855), Anti-ERK1/2 (#9102), Anti-phospho-T202/Y204-ERK1/2 (#4370), (1:1000, Cell Signaling Technlogy), Anti GAPDH (1:5000, Millipore).

Techniques: Expressing, Immunohistochemistry, Staining, Transduction

Journal: Oncotarget

Article Title: Anti-cancer effect of bee venom on colon cancer cell growth by activation of death receptors and inhibition of nuclear factor kappa B

doi:

Figure Lengend Snippet: A. Expression of apoptosis regulatory proteins related intrinsic pathway was determined by Western blotting analysis with antibodies against capase-3, caspase-8, capase-9, Bax, Bcl-2 and β-actin (internal control). B. Extrinsic pathway was determined by Western blotting with antibodies against Fas, DR3, DR4, DR5, TRAIL, p21, p53 and β-actin (internal control). Values under Western band indicate the density of band. Each band is representative for three experiments.

Article Snippet: Caspase-3, caspase-8, caspase-9 antibodies were purchased from Cell Signaling Technology Inc. (Beverly, MA).

Techniques: Expressing, Western Blot

Journal: Oncotarget

Article Title: Anti-cancer effect of bee venom on colon cancer cell growth by activation of death receptors and inhibition of nuclear factor kappa B

doi:

Figure Lengend Snippet: A. Expression of apoptosis regulatory proteins was detected by Western blotting using DR4, DR5, Bax, Bcl-2, caspase-3, caspase-8 and β-actin (internal control) in xenograft tumor samples (3 samples/each group) as described in materials and methods. B. DNA binding activity of NF-κB was determined by EMSA in nucleus extract from xenograft tumor samples (3 samples/each group) as described in materials and methods.

Article Snippet: Caspase-3, caspase-8, caspase-9 antibodies were purchased from Cell Signaling Technology Inc. (Beverly, MA).

Techniques: Expressing, Western Blot, Binding Assay, Activity Assay